ABSTRACT
ObjectiveIn order to compare the alteration of reelin-immunoreactive Cajal-Retzius cells (CR cells) in molecular layer of dentate gyrus of APPswe transgenic mice with wild type, the histochemical and developmental characteristics of CR cells were studied, therefore, the roles of CR cells in Alzheimer's disease would be revealed further.Methods The Thioflavine S staining, reelin immunofluorescence with or without reelin/glutamate and reelin/GABA immuno-double staining were carried out in the study. In the meantime, Western blotting was used to study the expression of reelin in hippocampi of the both wild type and transgenic mice. Results Reelin positive CR cells could be double-labeled with either glutamate or GABA immunostaining. Caspase-3 immunofluorescence demonstrated that some CR cells went through apoptosis during their development. Compared with wild type, CR cells in APPswe transgenic mice had significantly decreased in the molecular layer of the dentate gyrus. The result was supported with Western blotting analysis of reelin expression in hippocampus. Conclusion Reelin could be co-expressed with either glutamate or GABA, suggesting CR cells would be glutamatergic exciting neurons and GABAergic interneurons. The loss of CR cells during development probably was caused by the neuroapoptosis. Significant decrease of CR cells in hippocampus of APPswe transgenic mice indicated reelin may play an important role in AD pathological alterations.
ABSTRACT
Objective In order to investigate the influence of reelin deficiency on the hippocampal development, the histochemical characteristics of hippocampal pyramidal cells and granule cells of reeler mice were analyzed, therefore, reelin's function would be better understood with more morphological evidences. Methods With immunofluorescent double labeling, the pyramidal cells, granule cells and mossy cells in hippocampi between wild type and reeler mice were labeled. Results The development and lamination of hippocampal cortical plate were in disorder. Pyramidal cells and granule cells dispersed, and moreover, granule cells proliferated rapidly and migrated into hilus, so that the bound between granule layer and hilus disappeared. Conclusion As a stop signal and regulatory signal, reelin plays important roles in neuronal migration of developing cortical plate, especially in the regulation of granule cell proliferation.
ABSTRACT
The problems about scientific research design,data disposal and paper writing of medical scientific research are described in the article.
ABSTRACT
Objective To evaluate the Imaging diagnosis of SARS in death case.Methods 11 death patients of SARS in keeping with criteria from April to May 2003 were enrolled into the study. Imaging characteristics of chest x-ray film and CT in these patients were analysed.Results All cases were positive on chest X-ray film and CT,the lung shadows were marked.The extent of the pathological changes in lungs was 80%～90% in 6 cases and 90% above in 5 cases,multilobcs of bilateral lungs were involved in all cases.The lesions were diffuse spotty shadow or shaggy cloudy shadow in 8 cases,ground-glass opacification in one case and bronchogram structures could be seen.Conclusion Imaging findings in the cases with severe SARS mostly manifest as effusion,consolidation and interstitial inflammation in multilobes of bilateral lungs.
ABSTRACT
Objective To reseach variation of clinical and imaging in different stages of patients with SARS.Methods The author reviewed imaging findings and the clinical manifestations of 123 cases of SARS.Results Imaging changes (1)Early stage lesions were singular focus and multifocuses.Focuses exihibited multiform and multishape.The lung parenchyma not change evidently.(2)Progressive stage focuses developed rapidly,the extent enlarged,the number of focuses increased rapidly,multiform and multishape in imaging manifestations.(3)Absorb stage focuses evidently shruink,less dense and focal fibrosis.Clinical manifestation (1)Early stage the clinical manifestations were characterized by the onset of fever,associated with systemic and joint aching pains and dry coughs.Leucocyte counts showed normal or lower than normal. (2)Progressive stage these symptoms worsened and saturation degree of blood oxygen declined.20% patients developed ARDS.80% patients recoveried and leaved from hospital.Conclusion Imaging changes in patients with SARS took after the other pneumonia and may be indistinguishable from other conditions that result in airspace disease.But imaging findings offers an important diagnostic clue to SARS,and it is valuable in showing response to therapy and understanding state of an illness.